IGEM:UC Berkeley/2006/Ligation

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1. Create Ligation Mixture:<br>
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#Create Ligation Mixture:
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  * 5.5 uL water
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#*5.5 uL water
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  * 1 uL ligation buffer
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#*1 uL ligation buffer
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  * 0.5 uL ligase
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#*0.5 uL ligase
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  * 1 uL backbone (pAC997)
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#*1 uL backbone (pAC997)
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  * 2 uL insert <br>
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#*2 uL insert  
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#Let ligate at room temp for 30 minutes <br>
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2. Let ligate at room temp for 30 minutes <br>
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#*Note: For the control, add 2 uL of water instead of the insert
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Note: For the control, add 2 uL of water instead of the insert
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Revision as of 18:16, 19 June 2006

  1. Create Ligation Mixture:
    • 5.5 uL water
    • 1 uL ligation buffer
    • 0.5 uL ligase
    • 1 uL backbone (pAC997)
    • 2 uL insert
  2. Let ligate at room temp for 30 minutes
    • Note: For the control, add 2 uL of water instead of the insert
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