IGEM:UC Berkeley/2006/Ligation: Difference between revisions

From OpenWetWare
Jump to navigationJump to search
No edit summary
No edit summary
Line 1: Line 1:
1. Create Ligation Mixture:<br>
#Create Ligation Mixture:
  * 5.5 uL water
#*5.5 uL water
  * 1 uL ligation buffer
#*1 uL ligation buffer
  * 0.5 uL ligase
#*0.5 uL ligase
  * 1 uL backbone (pAC997)
#*1 uL backbone (pAC997)
  * 2 uL insert <br>
#*2 uL insert  
 
#Let ligate at room temp for 30 minutes <br>
2. Let ligate at room temp for 30 minutes <br>
#*Note: For the control, add 2 uL of water instead of the insert
Note: For the control, add 2 uL of water instead of the insert

Revision as of 15:16, 19 June 2006

  1. Create Ligation Mixture:
    • 5.5 uL water
    • 1 uL ligation buffer
    • 0.5 uL ligase
    • 1 uL backbone (pAC997)
    • 2 uL insert
  2. Let ligate at room temp for 30 minutes
    • Note: For the control, add 2 uL of water instead of the insert
Retrieved from "https://openwetware.org/mediawiki/index.php?title=IGEM:UC_Berkeley/2006/Ligation&oldid=43181"