IGEM:UC Berkeley/2006/Ligation: Difference between revisions
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#Let ligate at room temp for 30 minutes <br> | #Let ligate at room temp for 30 minutes <br> | ||
#*Note: For the control, add 2 uL of water instead of the insert | #*Note: For the control, add 2 uL of water instead of the insert | ||
Scaled-Up Ligations: | |||
*Use this for the construction of libraries. | |||
#69uL H2O | |||
#10uL Ligase buffer | |||
#10 uL backbone | |||
#10uL insert | |||
#1uL Ligase | |||
#Incubate at room temperature for 30 minutes. |
Revision as of 11:00, 26 June 2006
- Create Ligation Mixture:
- 5.5 uL water
- 1 uL ligation buffer
- 0.5 uL ligase
- 1 uL backbone (pAC997)
- 2 uL insert
- Let ligate at room temp for 30 minutes
- Note: For the control, add 2 uL of water instead of the insert
Scaled-Up Ligations:
- Use this for the construction of libraries.
#69uL H2O #10uL Ligase buffer #10 uL backbone #10uL insert #1uL Ligase
- Incubate at room temperature for 30 minutes.