IGEM:UC Berkeley/2006/Ligation

From OpenWetWare

< IGEM:UC Berkeley/2006
Revision as of 15:15, 14 June 2006 by Jenlu (Talk | contribs)
(diff) ←Older revision | Current revision (diff) | Newer revision→ (diff)
Jump to: navigation, search
1. Create Ligation Mixture:
* 5.5 uL water * 1 uL ligation buffer * 0.5 uL ligase * 1 uL backbone (pAC997) * 2 uL insert
Note: For the control, add 2 uL of water instead of the insert
Personal tools