IGEM:UC Berkeley/2006/Ligation

1. Create Ligation Mixture:
   • 5.5 uL water
   • 1 uL ligation buffer
   • 0.5 uL ligase
   • 1 uL backbone (pAC997)
   • 2 uL insert
2. Let ligate at room temp for 30 minutes
   
   • Note: For the control, add 2 uL of water instead of the insert