IGEM:UC Berkeley/2006/PCRPrep

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PCR Prep

  1. 38.5 ul water
  2. 5 ul buffer2
  3. 5 ul dNTP
  4. 1 ul oligo1
  5. 1 ul oligo2
  6. 1 ul DNA template
  7. .75 ul polymerase

50 ul total volume

Combine in PCR tube.

Notes:

To make 1ul oligo at correct concentration, take 1ul oligo and 9ul water, combine, transfer 1ul of solution to PCR tube

Keep polymerase on ice at all times

39 ul water can be used in place of 38.5ul water

-DLK_062106

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