IGEM:UC Berkeley/2006/Plasmid Transformation

From OpenWetWare

(Difference between revisions)
Jump to: navigation, search
Line 1: Line 1:
'''KCM-competent cell heat shock'''<br>
'''KCM-competent cell heat shock'''<br>
#create cocktail on ice
#create cocktail on ice
-
  20 uL TGI cells<br>
+
*20 uL TGI cells<br>
-
  10 uL KCM buffer<br>
+
*10 uL KCM buffer<br>
-
  70 uL water<br>
+
*70 uL water<br>
-
  1 uL plasmid<br>
+
*1 uL plasmid<br>
#heat shock for 1.5 min at 42 degrees C
#heat shock for 1.5 min at 42 degrees C
#incubate on ice for 5 min.
#incubate on ice for 5 min.
#plate
#plate

Revision as of 17:02, 9 June 2006

KCM-competent cell heat shock

  1. create cocktail on ice
  • 20 uL TGI cells
  • 10 uL KCM buffer
  • 70 uL water
  • 1 uL plasmid
  1. heat shock for 1.5 min at 42 degrees C
  2. incubate on ice for 5 min.
  3. plate
Personal tools