IGEM:UC Berkeley/2006/Plasmid Transformation

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'''KCM-competent cell heat shock'''<br>
'''KCM-competent cell heat shock'''<br>
#create cocktail on ice
#create cocktail on ice
-
*20 uL TGI cells<br>
+
  20 uL TGI cells<br>
-
*10 uL KCM buffer<br>
+
  10 uL KCM buffer<br>
-
*70 uL water<br>
+
  70 uL water<br>
-
*1 uL plasmid<br>
+
  1 uL plasmid<br>
#heat shock for 1.5 min at 42 degrees C
#heat shock for 1.5 min at 42 degrees C
#incubate on ice for 5 min.
#incubate on ice for 5 min.
#plate
#plate

Revision as of 17:03, 9 June 2006

KCM-competent cell heat shock

  1. create cocktail on ice
 20 uL TGI cells
10 uL KCM buffer
70 uL water
1 uL plasmid
  1. heat shock for 1.5 min at 42 degrees C
  2. incubate on ice for 5 min.
  3. plate
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