IGEM:UC Berkeley/2006/Plasmid Transformation: Difference between revisions
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'''KCM-competent cell heat shock'''<br> | '''KCM-competent cell heat shock'''<br> | ||
#create cocktail on ice | #create cocktail on ice | ||
20 uL TGI cells<br> | |||
10 uL KCM buffer<br> | |||
70 uL water<br> | |||
1 uL plasmid<br> | |||
#heat shock for 1.5 min at 42 degrees C | #heat shock for 1.5 min at 42 degrees C | ||
#incubate on ice for 5 min. | #incubate on ice for 5 min. | ||
#plate | #plate | ||
Revision as of 14:03, 9 June 2006
KCM-competent cell heat shock
- create cocktail on ice
20 uL TGI cells
10 uL KCM buffer
70 uL water
1 uL plasmid
- heat shock for 1.5 min at 42 degrees C
- incubate on ice for 5 min.
- plate
