IGEM:UC Berkeley/2006/Plasmid Transformation

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'''KCM-competent cell heat shock'''<br>
'''KCM-competent cell heat shock'''<br>
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#create cocktail on ice
+
1. create cocktail on ice<br>
   20 uL TGI cells<br>
   20 uL TGI cells<br>
   10 uL KCM buffer<br>
   10 uL KCM buffer<br>
   70 uL water<br>
   70 uL water<br>
   1 uL plasmid<br>
   1 uL plasmid<br>
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#heat shock for 1.5 min at 42 degrees C
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2. heat shock for 1.5 min at 42 C <br>
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#incubate on ice for 5 min.
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3. incubate on ice for 5 min. <br>
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#plate
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4. plate <br>
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-JL_06/09/06

Revision as of 16:05, 9 June 2006

KCM-competent cell heat shock
1. create cocktail on ice

 20 uL TGI cells
10 uL KCM buffer
70 uL water
1 uL plasmid

2. heat shock for 1.5 min at 42 C
3. incubate on ice for 5 min.
4. plate
-JL_06/09/06

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