IGEM:UC Berkeley/2006/Plasmid Transformation

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  3. incubate on ice for 5 min. <br>
  3. incubate on ice for 5 min. <br>
  4. plate <br>
  4. plate <br>
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-JL_06/09/06
+
-JL_06/09/06 <br>
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'''Transformation of Ligation mix'''
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1. Create cocktail on ice<br>
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  *100 uL TGI cells<br>
 +
  *15 uL KCM buffer<br>
 +
  *25 uL water<br>
 +
  *10 uL plasmid<br>
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2. Heat shock for 1.5 min at 42 C <br>
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3. Rescue with SOB media
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4. Incubate in 37 C shaker for 1 hour
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5. Plate
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-JL_06/14/06

Revision as of 14:09, 14 June 2006

KCM-competent cell heat shock

1. create cocktail on ice
*20 uL TGI cells
*10 uL KCM buffer
*70 uL water
*1 uL plasmid
2. heat shock for 1.5 min at 42 C
3. incubate on ice for 5 min.
4. plate

-JL_06/09/06

Transformation of Ligation mix

1. Create cocktail on ice
*100 uL TGI cells
*15 uL KCM buffer
*25 uL water
*10 uL plasmid
2. Heat shock for 1.5 min at 42 C
3. Rescue with SOB media 4. Incubate in 37 C shaker for 1 hour 5. Plate

-JL_06/14/06

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