IGEM:UC Berkeley/2006/Plasmid Transformation

From OpenWetWare

(Difference between revisions)
Jump to: navigation, search
Line 9: Line 9:
  4. plate <br>
  4. plate <br>
-JL_06/09/06 <br>
-JL_06/09/06 <br>
-
 
+
<br>
'''Transformation of Ligation mix'''
'''Transformation of Ligation mix'''
  1. Create cocktail on ice<br>
  1. Create cocktail on ice<br>

Revision as of 14:10, 14 June 2006

KCM-competent cell heat shock

1. create cocktail on ice
*20 uL TGI cells
*10 uL KCM buffer
*70 uL water
*1 uL plasmid
2. heat shock for 1.5 min at 42 C
3. incubate on ice for 5 min.
4. plate

-JL_06/09/06

Transformation of Ligation mix

1. Create cocktail on ice
*100 uL TGI cells
*15 uL KCM buffer
*25 uL water
*10 uL plasmid
2. Heat shock for 1.5 min at 42 C
3. Rescue with SOB media 4. Incubate in 37 C shaker for 1 hour 5. Plate

-JL_06/14/06

Personal tools