IGEM:UC Berkeley/2006/Plasmid Transformation

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Line 17: Line 17:
   *10 uL plasmid<br>
   *10 uL plasmid<br>
  2. Heat shock for 1.5 min at 42 C <br>
  2. Heat shock for 1.5 min at 42 C <br>
-
  3. Rescue with SOB media  
+
  3. Rescue with SOB media <br>
-
  4. Incubate in 37 C shaker for 1 hour
+
  4. Incubate in 37 C shaker for 1 hour<br>
-
  5. Plate
+
  5. Plate<br>
-JL_06/14/06
-JL_06/14/06

Revision as of 14:11, 14 June 2006

KCM-competent cell heat shock

1. create cocktail on ice
*20 uL TGI cells
*10 uL KCM buffer
*70 uL water
*1 uL plasmid
2. heat shock for 1.5 min at 42 C
3. incubate on ice for 5 min.
4. plate

-JL_06/09/06

Transformation of Ligation mix

1. Create cocktail on ice
*100 uL TGI cells
*15 uL KCM buffer
*25 uL water
*10 uL plasmid
2. Heat shock for 1.5 min at 42 C
3. Rescue with SOB media
4. Incubate in 37 C shaker for 1 hour
5. Plate

-JL_06/14/06

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