IGEM:UC Berkeley/2006/Plasmid Transformation: Difference between revisions
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'''KCM-competent cell heat shock'''<br> | '''KCM-competent cell heat shock'''<br> | ||
1. create cocktail on ice<br> | |||
20 uL TGI cells<br> | 20 uL TGI cells<br> | ||
10 uL KCM buffer<br> | 10 uL KCM buffer<br> | ||
70 uL water<br> | 70 uL water<br> | ||
1 uL plasmid<br> | 1 uL plasmid<br> | ||
2. heat shock for 1.5 min at 42 C <br> | |||
3. incubate on ice for 5 min. <br> | |||
4. plate <br> | |||
-JL_06/09/06 | |||
Revision as of 14:05, 9 June 2006
KCM-competent cell heat shock
1. create cocktail on ice
20 uL TGI cells
10 uL KCM buffer
70 uL water
1 uL plasmid
2. heat shock for 1.5 min at 42 C
3. incubate on ice for 5 min.
4. plate
-JL_06/09/06
