IGEM:UC Berkeley/2006/Plasmid Transformation: Difference between revisions
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'''KCM-competent cell heat shock'''<br> | '''KCM-competent cell heat shock'''<br> | ||
1. create cocktail on ice<br> | 1. create cocktail on ice<br> | ||
*20 uL TGI cells<br> | |||
*10 uL KCM buffer<br> | |||
*70 uL water<br> | |||
*1 uL plasmid<br> | |||
2. heat shock for 1.5 min at 42 C <br> | 2. heat shock for 1.5 min at 42 C <br> | ||
3. incubate on ice for 5 min. <br> | 3. incubate on ice for 5 min. <br> | ||
4. plate <br> | 4. plate <br> | ||
-JL_06/09/06 | -JL_06/09/06 | ||
Revision as of 14:06, 9 June 2006
KCM-competent cell heat shock
1. create cocktail on ice
*20 uL TGI cells
*10 uL KCM buffer
*70 uL water
*1 uL plasmid
2. heat shock for 1.5 min at 42 C
3. incubate on ice for 5 min.
4. plate
-JL_06/09/06
