IGEM:UC Berkeley/2006/Plasmid Transformation

KCM-competent cell heat shock

1. create cocktail on ice

  20 uL TGI cells

  10 uL KCM buffer

  70 uL water

  1 uL plasmid

1. heat shock for 1.5 min at 42 degrees C
2. incubate on ice for 5 min.
3. plate