IGEM:UC Berkeley/2006/Plasmid Transformation

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KCM-competent cell heat shock

  1. create cocktail on ice
  • 20 uL TGI cells
  • 10 uL KCM buffer
  • 70 uL water
  • 1 uL plasmid
  1. heat shock for 1.5 min at 42 degrees C
  2. incubate on ice for 5 min.
  3. plate
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