IGEM:UC Berkeley/2006/Plasmid Transformation
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KCM-competent cell heat shock
- create cocktail on ice
20 uL TGI cells
10 uL KCM buffer
70 uL water
1 uL plasmid
- heat shock for 1.5 min at 42 degrees C
- incubate on ice for 5 min.
- plate