IGEM:UC Berkeley/2006/Plasmid Transformation

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KCM-competent cell heat shock
1. create cocktail on ice

 20 uL TGI cells
10 uL KCM buffer
70 uL water
1 uL plasmid

2. heat shock for 1.5 min at 42 C
3. incubate on ice for 5 min.
4. plate
-JL_06/09/06

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