IGEM:UC Berkeley/2006/Plasmid Transformation

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KCM-competent cell heat shock

1. create cocktail on ice
*20 uL TGI cells
*10 uL KCM buffer
*70 uL water
*1 uL plasmid
2. heat shock for 1.5 min at 42 C
3. incubate on ice for 5 min.
4. plate

-JL_06/09/06

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