IGEM:UC Berkeley/2006/Sequencing: Difference between revisions
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1. Volumes to add: | 1. Volumes to add:<br> | ||
*5 uL of plasmid | *5 uL of plasmid | ||
*3.2 uL of 1 uM sequencing primer | *3.2 uL of 1 uM sequencing primer | ||
*4.8 uL of water | *4.8 uL of water | ||
total= 13 uL | total= 13 uL | ||
2. Print 2 copies | 2. Label constructs iG1,iG2, etc.<br> | ||
3. Record actual plasmid name correlating to iG1, iG2, etc.<br> | |||
4. Print 2 copies (one for the sequencing people and one for Rumi)<br> | |||
-JL_06/14/06 | |||
Revision as of 11:51, 14 June 2006
1. Volumes to add:
*5 uL of plasmid *3.2 uL of 1 uM sequencing primer *4.8 uL of water total= 13 uL 2. Label constructs iG1,iG2, etc.
3. Record actual plasmid name correlating to iG1, iG2, etc.
4. Print 2 copies (one for the sequencing people and one for Rumi)
-JL_06/14/06
