IGEM:UC Berkeley/2006/Sequencing
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< IGEM:UC Berkeley/2006(Difference between revisions)
Current revision (14:51, 14 June 2006) (view source) |
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*3.2 uL of 1 uM sequencing primer | *3.2 uL of 1 uM sequencing primer | ||
*4.8 uL of water | *4.8 uL of water | ||
| - | total= 13 uL | + | total= 13 uL<br> |
2. Label constructs iG1,iG2, etc.<br> | 2. Label constructs iG1,iG2, etc.<br> | ||
3. Record actual plasmid name correlating to iG1, iG2, etc.<br> | 3. Record actual plasmid name correlating to iG1, iG2, etc.<br> | ||
Current revision
1. Volumes to add:
*5 uL of plasmid *3.2 uL of 1 uM sequencing primer *4.8 uL of water total= 13 uL
2. Label constructs iG1,iG2, etc.
3. Record actual plasmid name correlating to iG1, iG2, etc.
4. Print 2 copies (one for the sequencing people and one for Rumi)
-JL_06/14/06
