IGEM:UC Berkeley/2006/TlambdaOlambda

-Start with saturated culture
-Wash out all antibiotics

 1. pour 1 mL of culture into 1.5 mL eppendorf tube
 2. spin (10 sec. at full speed)
 3. dump out residue
 4. pipette 1 mL of LB 
 5. resuspend (by vortexing)
 6. spin (10 sec. at full speed)
 7. dump out residue
 8. pipette 1mL of LB 
 9. resuspend (by vortexing)

-setup:

 1. pipette 50uL recipient (ie. EC100D) and 50 uL donor into eppendorf
 2. mix and incubate (1 hour @ 37C)
 3. add 1mL LB 
 4. mix
 5. plate 25 uL on triA or triK

--Jenlu 17:34, 26 June 2006 (EDT)