IGEM:UNAM-Genomics-Mexico/2009/Notebook/iGEM 2011 UNAM-Genomics Mexico/2011/06/23: Difference between revisions
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(Autocreate 2011/06/23 Entry for IGEM:UNAM-Genomics-Mexico/2009/Notebook/iGEM_2011_UNAM-Genomics_Mexico) |
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== | ==Regrowing PLasmid PBBRMCS-5 in E. coli BL21-DE3== | ||
2 tubes with 5 mL of liquid LB and 5 μL of gentamicin with E. coli BL21-DE3 containing the Plasmid PBBRMCS-5. | |||
1 tube with 5 mL of liquid LB and 5 μL of gentamicin as a control. | |||
Leave for 12 hours and the extract the plasmid. | |||
Revision as of 09:22, 23 June 2011
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Regrowing PLasmid PBBRMCS-5 in E. coli BL21-DE32 tubes with 5 mL of liquid LB and 5 μL of gentamicin with E. coli BL21-DE3 containing the Plasmid PBBRMCS-5. 1 tube with 5 mL of liquid LB and 5 μL of gentamicin as a control. Leave for 12 hours and the extract the plasmid.
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