IGEM:UNAM-Genomics Mexico/2009/Notebook/Collaborations/2010/09/20

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(Autocreate 2010/09/20 Entry for IGEM:UNAM-Genomics_Mexico/2009/Notebook/Collaborations)
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==Entry title==
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==Mail from Chris French: parts sent==
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* Insert your content here.
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Dear Luis,
 +
 
 +
I've just put the BioBricks in the mail. The sequence of lumP is 
 +
slightly different from the one in the Registry as it comes from a 
 +
different strain. I'll send it to you later - today's a bit busy for 
 +
me. I've also included a new cph8 (red light sensor) clone that I just 
 +
completed, though it has not been sequence-verified yet (there were 
 +
several errors that needed to be corrected). I'm hoping to get the 
 +
sequence results back on Wednesday, so I'll let you know then if it's 
 +
OK. We're about to start assembling the complete red light sensor 
 +
system with a reporter - probably will use YFP or RFP rather than GFP 
 +
as we get lower backgrounds with these in my fluorimeter.
 +
 
 +
BTW, sorry if this is out if line, but I'm just getting bits and 
 +
pieces of second hand information - I have somehow received the 
 +
impression, rightly or wrongly, that your team has been having some 
 +
issues getting things into pSB1C3. If this is the case, and if we are 
 +
successful in cloning the PCR products you sent us in pSB1C3, would 
 +
you like us to send you the plasmids for submission? or is this now 
 +
under control, or have I misunderstood the situation?
 +
 
 +
best wishes,
 +
 
 +
Chris F.
 +
 
 +
 

Revision as of 10:14, 20 September 2010

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Mail from Chris French: parts sent

Dear Luis,

I've just put the BioBricks in the mail. The sequence of lumP is slightly different from the one in the Registry as it comes from a different strain. I'll send it to you later - today's a bit busy for me. I've also included a new cph8 (red light sensor) clone that I just completed, though it has not been sequence-verified yet (there were several errors that needed to be corrected). I'm hoping to get the sequence results back on Wednesday, so I'll let you know then if it's OK. We're about to start assembling the complete red light sensor system with a reporter - probably will use YFP or RFP rather than GFP as we get lower backgrounds with these in my fluorimeter.

BTW, sorry if this is out if line, but I'm just getting bits and pieces of second hand information - I have somehow received the impression, rightly or wrongly, that your team has been having some issues getting things into pSB1C3. If this is the case, and if we are successful in cloning the PCR products you sent us in pSB1C3, would you like us to send you the plasmids for submission? or is this now under control, or have I misunderstood the situation?

best wishes,

Chris F.




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