IGEM:UNAM-Genomics Mexico/2009/Notebook/H2/2011/07/28: Difference between revisions

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===Further work===
===Further work===
Digest construction 2 and prepare for insertion.
''Determine best outcome, insert C2 into C1's plamid or vice versa.''
Ligate construction 1 + construction 2 + BBa_b0015(dual terminator)
Ligate construction 1 + construction 2 + BBa_b0015(dual terminator)


Revision as of 21:40, 28 July 2011

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Green Light Receptor

Goal: prepare two constructions for ligation. Results: in preparation

Construction 1

Contents: BBa_J23117(promoter) + RBS + CcaR + CcaS

Work done: duplicate plasmid extraction with Kit; duplicate plasmid restriction with XbaI & PstI.

Restriction mixture (40μL total):

  1. 15μL DNA
  2. 1μL BSA
  3. 4μL Buffer #2
  4. 2μL Enzyme PstI
  5. 2μL Enzyme XbaI
  6. 16μL H2O

Construction 2

Contents: PcyA + Ho1

Work done: culture undergoing re-growth as colonies were too small.

Further work

Digest construction 2 and prepare for insertion.

Determine best outcome, insert C2 into C1's plamid or vice versa.

Ligate construction 1 + construction 2 + BBa_b0015(dual terminator)


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