IGEM:UNAM-Genomics Mexico/2009/Notebook/H2 Pae/2011/08/22: Difference between revisions
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2. Once ready a I put the mix into the thermocylcer, the first cycle was: | |||
94°C/5min | |||
3. Then I added the mix 2, after that the following cycle were done: | |||
30 cylces: | |||
94°C / 30 seg | |||
60°C / 30 seg | |||
72°C / 3 min | |||
1 cycle: | |||
72°C / 5 min | |||
4° C / ∞ | |||
I will see the results on wednesday, in an electrophoresis gel. | |||
Revision as of 10:44, 27 August 2011
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Abstract
Parts assemblyToday I did a PCR from the pSB1C3 plasmid amplifying all the backbone, because I will need it to isert our parts. I did the PCR with the RTTH enzyme, according to the following steps. 1. I prepared the two mixes, which were: Mix 1
Mix 2
2. Once ready a I put the mix into the thermocylcer, the first cycle was: 94°C/5min 3. Then I added the mix 2, after that the following cycle were done: 30 cylces: 94°C / 30 seg 60°C / 30 seg 72°C / 3 min 1 cycle: 72°C / 5 min 4° C / ∞ I will see the results on wednesday, in an electrophoresis gel.
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