IGEM:UNAM-Genomics Mexico/2009/Notebook/H2 Pae/2011/09/02: Difference between revisions
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The remaining lanes are not of interest for this analysis | The remaining lanes are not of interest for this analysis | ||
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The PCR is not visible in this gel but a I think its not enough concetration to see it, however a problem could have arise during the PCR cleaning | The PCR is not visible in this gel but a I think its not enough concetration to see it, however a problem could have arise during the PCR cleaning | ||
Revision as of 15:47, 2 September 2011
 iGEM Project name 1
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Abstract
MainStandardizationDigestion results In the following electrophoresis gel (120V X 45min) Lanes: 1. Ladder 500 bp. 2. PCR Digestion. 3. pBBR1MCS-5 digestion. 4. pBBR1MCS-5 plasmid The remaining lanes are not of interest for this analysis
The PCR is not visible in this gel but a I think its not enough concetration to see it, however a problem could have arise during the PCR cleaning Ligation Regardless the problem of the PCR in the gel I did 3 ligations with these amounts:
I left the rections 5 hours
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