IGEM:UNAM-Genomics Mexico/2009/Notebook/HRG personal log/2011/05/21: Difference between revisions
Helena Reyes (talk | contribs) (Autocreate 2011/05/21 Entry for IGEM:UNAM-Genomics_Mexico/2009/Notebook/HRG_personal_log) |
Helena Reyes (talk | contribs) (Log about the day in the Lab uploaded) |
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==Entry title== | ==Entry title== | ||
21/05/2011 06:04 a.m. | |||
I have had little experience in the wet lab. This is the first time I encounter myself in the laboratory alone. | |||
Before taking the gel out, I must know, which is the aim of this gel? (I did not prepare de DNA with which it was loaded and it is not stated in the notebook) I decided to look at the concentration of the gel | |||
I asked as many people as I could find at this time of the morning, and I decided to take the gel out and place it on the transilluminator | |||
The results are as follows: | |||
Lane 1 Ladder (1Kb Fermentas) Ladder | |||
Lane 2 Forward Negative | |||
Lane 3 Reverse Negative | |||
Lane 4 No DNA Negative | |||
Lane 5 No oligos Negative | |||
Lane 6 5mg DNA Band | |||
Lane 7 10mg DNA Band | |||
Lane 8 Ladder (1Kb Fermentas) Ladder | |||
The expectations were those, concordantly , the outcome is a success. | |||
Revision as of 13:28, 21 May 2011
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Entry title21/05/2011 06:04 a.m. I have had little experience in the wet lab. This is the first time I encounter myself in the laboratory alone. Before taking the gel out, I must know, which is the aim of this gel? (I did not prepare de DNA with which it was loaded and it is not stated in the notebook) I decided to look at the concentration of the gel I asked as many people as I could find at this time of the morning, and I decided to take the gel out and place it on the transilluminator The results are as follows: Lane 1 Ladder (1Kb Fermentas) Ladder Lane 2 Forward Negative Lane 3 Reverse Negative Lane 4 No DNA Negative Lane 5 No oligos Negative Lane 6 5mg DNA Band Lane 7 10mg DNA Band Lane 8 Ladder (1Kb Fermentas) Ladder The expectations were those, concordantly , the outcome is a success.
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