IGEM:UNAM/2009/Notebook/IGEM project/2011/05/30: Difference between revisions
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'''Abstract''' | '''Abstract''' | ||
Today I | Today I did a gel of the DNA plasmid PBBMRCS-5, the gel showed that the plasmid was cleaned of chromosome. | ||
*1. I did the agar to 1%. | *1. I did the agar to 1%. |
Revision as of 11:17, 28 June 2011
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Gel of PBBMRCS-5 plasmidAbstract Today I did a gel of the DNA plasmid PBBMRCS-5, the gel showed that the plasmid was cleaned of chromosome.
Note: PBBMRCS-5 was extracted using alcaline lysis and 15μL Plasmid DNA digested with XBA I and Hind III. PBBRMCS-5 Plasmid Digestion
Leave all night at 37ºC in agitation.
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