IGEM:UNAM/2009/Notebook/IGEM project/2011/06/27: Difference between revisions
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(Autocreate 2011/06/27 Entry for IGEM:UNAM/2009/Notebook/IGEM_project) |
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== | ==Grow of ''Chlamydomonas reinhartii wild type''== | ||
* | * Today I grew ''Chlamydomonas reinhardtii'' in liquid medium. | ||
Elenmeyer Flask -> Tap medium (50 ml)with Amp (50 μl) + ''Chlamydomonas reinhardtii'' (Miriam's tube). | |||
Tube Control -> Tap medium (5 ml)with Amp (5 μl) | |||
Tube 1 -> Tap medium (5 ml)with Amp (5 μl) + ''Chlamydomonas reinhardtii'' (petri dish 14/jun/2011). | |||
Tube 2 -> Tap medium (5 ml)with Amp (5 μl) + ''Chlamydomonas reinhardtii'' (petri dish 16/jun/2011). | |||
Pablo found an article which authors put 100 μg/ml of Amp with the purpose of avoiding pollution by bacterias. So, we did other Elenmeyer flask with the proportions of 500 μl of Ampicillin in 100 ml of TAP medium. | |||
Elenmeyer Flask 2 -> Tap medium (50 ml)with Amp (250 μl) + ''Chlamydomonas reinhardtii'' (Miriam's tube). | |||
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Revision as of 08:55, 28 June 2011
 iGEM Project name 1
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Grow of Chlamydomonas reinhartii wild type
Elenmeyer Flask -> Tap medium (50 ml)with Amp (50 μl) + Chlamydomonas reinhardtii (Miriam's tube). Tube Control -> Tap medium (5 ml)with Amp (5 μl) Tube 1 -> Tap medium (5 ml)with Amp (5 μl) + Chlamydomonas reinhardtii (petri dish 14/jun/2011). Tube 2 -> Tap medium (5 ml)with Amp (5 μl) + Chlamydomonas reinhardtii (petri dish 16/jun/2011). Pablo found an article which authors put 100 μg/ml of Amp with the purpose of avoiding pollution by bacterias. So, we did other Elenmeyer flask with the proportions of 500 μl of Ampicillin in 100 ml of TAP medium. Elenmeyer Flask 2 -> Tap medium (50 ml)with Amp (250 μl) + Chlamydomonas reinhardtii (Miriam's tube).
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