IGEM:UNAM/2009/Notebook/Modeling logbook Claudia/2010/08/19: Difference between revisions
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|style="background-color: #EEE"|[[Image: | |style="background-color: #EEE"|[[Image:TeamLogo.jpg|350px]]<span style="font-size:19px;"> UNAM-Genomics-Mexico team</span> | ||
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Revision as of 14:18, 9 October 2010
 UNAM-Genomics-Mexico team
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Working on LuxY synthesized plasmid from Mr.GeneIn order to test the correct lenght LuxY transformed, I am going to do a PCR reaction using as template the plasmid transformed harboring LuxY and the restriction fragments obtained with said plasmid. Primers: Forward:Preffix primer Reverse:Suffix primer
Results LuxY PCRs reactions:The product obtained from the two PCR reactions was around 2000 nt of size. Considering that the correct size is 820 nt, we are going to repeat the transformation of the plasmid, it's possible that we did a mistake and chosen the wrong plasmid. I'm going to do another PCR reaction using as template the plasmid from Mr. Gene that harbors LuxY.
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