IGEM:UNAM/2009/Notebook/Modeling logbook Claudia/2010/09/02: Difference between revisions
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|style="background-color: #EEE"|[[Image: | |style="background-color: #EEE"|[[Image:TeamLogo.jpg|350px]]<span style="font-size:19px;"> UNAM-Genomics-Mexico team</span> | ||
|style="background-color: #F2F2F2" align="center"|<html><img src="/images/9/94/Report.png" border="0" /></html> [[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>[[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]]<html> </html>}}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]]<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>}} | |style="background-color: #F2F2F2" align="center"|<html><img src="/images/9/94/Report.png" border="0" /></html> [[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>[[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]]<html> </html>}}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]]<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>}} | ||
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Revision as of 14:21, 9 October 2010
UNAM-Genomics-Mexico team | <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page <html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html> </html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html> | ||||||||||||||||||||||||||||||||||
Working on cI inverter construction, pSB3K3 backbone and E0240 partOnce the plasmids harboring the parts showed in the next table were correctly isolated, I am going to digest some with EcoRI and PstI and the others with XbaI and PstI in order to fuse them with their corresponding part.
K098991 preparation for ligation with plasmid pSB3K3 and J04450 preparation for ligation with promoters-LovTAP, trpL-cI inverter and trpL-GFP constructions for characterization. Plasmids used: Plasmid harboring K098991 isolated from colony 8. Plasmid harboring J04450 isolated from colony 8. Two replicas
P0451 and E0240 preparation for ligation with constitutive promoter (J23101) and trpL promoter Plasmids used: Plasmid harboring P0451 isolated from colony 6. Plasmid harboring E0240 isolated from colony 5. Two replicas
Note: Inactivate the enzymes at 80°C during 10 min. Results restriction enzyme assaysThe sizes of the digested products are around the expected lenght for each part.
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