IGEM:UNAM/2009/Notebook/Modeling logbook Claudia/2010/09/17: Difference between revisions
No edit summary |
|||
Line 1: | Line 1: | ||
{|{{table}} width="800" | {|{{table}} width="800" | ||
|- | |- | ||
|style="background-color: #EEE"|[[Image: | |style="background-color: #EEE"|[[Image:TeamLogo.jpg|350px]]<span style="font-size:19px;"> UNAM-Genomics-Mexico team</span> | ||
|style="background-color: #F2F2F2" align="center"|<html><img src="/images/9/94/Report.png" border="0" /></html> [[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>[[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]]<html> </html>}}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]]<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>}} | |style="background-color: #F2F2F2" align="center"|<html><img src="/images/9/94/Report.png" border="0" /></html> [[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>[[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]]<html> </html>}}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]]<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>}} | ||
|- | |- |
Revision as of 14:24, 9 October 2010
UNAM-Genomics-Mexico team | <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page <html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html> </html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html> |
Ligation Procedure:Lumazine/LuxY with plasmid pSB1C3 and promoters J23101/J23102.I'm working in Step 7 of the Ligation Procedure: Lumazine/LuxY with plasmid pSB1C3 and promoters J23101/J23102.
Analyze the colonies with Colony PCR to confirm that they contain the correct ligation. The primers that I am using are: Forward (5'->3'): Preffix primer. Reverse:(5'->3'): Suffix primer. These primers would amplify Lumazine/LuxY ligated to each promoter, if the ligation was correctly done. Results:Ligations Lumazine/LuxY with plasmid pSB1C3 and promoters J23101/J23102.Procedure Step 7After re-culturing the colonies transformed with each ligation mixture, only colonies harboring the Lumazine/LuxY Mr.gene constructions fused to J23101 and J23102 grew correctly.
Lumazine + J23101 ligation from colonies 1,5 and 15. Lumazine + J23102 ligation from colony 1,6 and 12. LuxY + J23101 ligation from colony 1, 2 and 15. LuxY + J23102 ligation from colony 2,4 and 5.
|