IGEM:UNAM/2009/Notebook/Modeling logbook Claudia/2010/10/02: Difference between revisions
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|style="background-color: #EEE"|[[Image: | |style="background-color: #EEE"|[[Image:TeamLogo.jpg|350px]]<span style="font-size:19px;"> UNAM-Genomics-Mexico team</span> | ||
|style="background-color: #F2F2F2" align="center"|<html><img src="/images/9/94/Report.png" border="0" /></html> [[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>[[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]]<html> </html>}}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]]<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>}} | |style="background-color: #F2F2F2" align="center"|<html><img src="/images/9/94/Report.png" border="0" /></html> [[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>[[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]]<html> </html>}}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]]<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>}} | ||
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Revision as of 14:26, 9 October 2010
UNAM-Genomics-Mexico team | <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page <html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html> </html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html> |
Ligation Procedure:Lumazine/LuxY and P0451 with promoter J23102.I'm working in Step 7 of the Ligation Procedure: Lumazine, LuxY and P0451 with promoter J23102.
Analyze the colonies with Colony PCR to confirm that they contain the correct ligation. The primers that I am using are: Forward (5'->3'): Preffix primer. Reverse:(5'->3'): Suffix primer. These primers would amplify Lumazine, LuxY and P0451 ligated to each promoter, if the ligations were correctly done. Results:Ligations Lumazine, LuxY and P0451 with promoter J23102.Procedure Step 7
P0451 + J23102 ligation from colonies 1,2 and 31.
Lumazine + J23102 ligation from colony 2,3,4,6 and 7. Ligation Procedure:Lumazine/LuxY and P0451 with promoter J23102.I'm working in Step 7 of the Ligation Procedure: cI inverter construction with pSB1C3 backbone.
Analyze the colonies with Colony PCR to confirm that they contain the correct ligation. The primers that I am using are: Forward (5'->3'): Preffix primer. Reverse:(5'->3'): Suffix primer. These primers would amplify the cI inverter (Parts P0451 + K098991) inside plasmid pSB1C3, if the ligation was correctly done. Results: Ligation Procedure: cI inverter(P0451 + K098991) with plasmid pSB1C3. Step 7
cI inverter(P0451 + K098991) with plasmid pSB1C3 ligation from colonies 2,3,4,5 and 6.
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