IGEM:UNAM Genomics Mexico/2009/Notebook/Wifi coli/2010/02/12: Difference between revisions
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(Autocreate 2010/02/12 Entry for IGEM:UNAM_Genomics_Mexico/2009/Notebook/Wifi_coli) |
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== | ==February 12th 2010 == | ||
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===Wi-Fi coli: Experimental and Modeling design= | |||
====Objective==== | |||
*Get an idea of final construction. | |||
====Looking for:==== | |||
*Assembly of all necessary bio-bricks (are they already assembled?) | |||
*Looking for bio-bricks and methods | |||
*Protocols (strain, reactants, medium) | |||
*We need to prove if it is possible for a luciferase emitter to stimulate a chromophore receptor. | |||
*Plasmid structure | |||
*Vectors must have the same structure, this structure is an iGEM standard, all bio-bricks have the same standard unless specified. | |||
*Standard assembling | |||
''Bio-bricks must contain:'' | |||
*Suffix and Prefix: | |||
These are sequences placed at both sides of the sequence to be inserted; these sequences contain several restriction sites in order to excise the bio-brick if necessary. | |||
It is important to notice that the principal sequence placed between the suffix and prefix sequences must lack restrictions sites contained in suffix/prefix sequences to avoid bio-brick damaging. | |||
*Origin of replication | |||
*Expressed sequence | |||
RBS (Ribosome binding site) | |||
Promoter | |||
Gene | |||
Terminus | |||
*Resistance gene | |||
The sequence to be expressed should be like this: | |||
Suffix--rbs—promoter—ATG—prot—stop codon--prefix | |||
*We need to search for bio-brick’s specifications like kind of restriction-sites, resistance gene, bio-brick’s length, etc. | |||
===Modeling=== | |||
''It is necessary to model:'' | |||
*Signal input | |||
*Transcriptional response after receptor stimulation depending on the number of photons received. | |||
*How strong was the transcriptional response after a defined photon input. | |||
*What do we expect of our model? | |||
*Modeling signal input and transcriptional response. | |||
*We need to model: elements, circuit, parameters, logic design. | |||
Revision as of 22:32, 24 May 2010
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February 12th 2010==Wi-Fi coli: Experimental and Modeling designObjective
Looking for:
Bio-bricks must contain:
These are sequences placed at both sides of the sequence to be inserted; these sequences contain several restriction sites in order to excise the bio-brick if necessary. It is important to notice that the principal sequence placed between the suffix and prefix sequences must lack restrictions sites contained in suffix/prefix sequences to avoid bio-brick damaging.
RBS (Ribosome binding site) Promoter Gene Terminus
The sequence to be expressed should be like this: Suffix--rbs—promoter—ATG—prot—stop codon--prefix
ModelingIt is necessary to model:
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