IGEM:UNAM Genomics Mexico/2009/Notebook/Wifi coli/2010/05/04

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(To get unidirectionality we purpose a system with optical fiber and aisled systems.)
Current revision (15:35, 11 May 2010) (view source)
(Modeling)
 
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LuM= Number of moles, which react in measure, this is by colony.
LuM= Number of moles, which react in measure, this is by colony.
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'''Whit this formula we can achieve the values for light emission and efficiency of our system.'''
+
'''With this formula we can achieve the values for light emission and efficiency of our system.'''
We need:
We need:
*Get values of K_cat.
*Get values of K_cat.

Current revision

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Modeling & WetLab Meetings

Modeling

Stoichiometry of Luciferase activity.

a[Luc] + b[ Luc-ina] + c[02] + d[ATP] -> z[Luc] + y [oxyLuc-ina] + w[C02] + u[AMP] + v[PPi]

We use that formula to know which is the limitant reactant and the optimum concentration.

The next figure is to show the posible conectreations of Luciferin and Luciferase. Image:Model.4.May.2010.jpg

To achieve a controlled model we use single copy plasmid or low copy number.

LuM= Kc_cat * LUC * (Scyt/(Km+Scyt)) *RLV

K_cat= [Constant of Activity]

LUC= [Luciferase]

Scyt = [Cytosolic Luciferase]

Km= Michaelis-Menten constant.

RLU= Relative Light Unit

LuM= Number of moles, which react in measure, this is by colony.

With this formula we can achieve the values for light emission and efficiency of our system. We need:

  • Get values of K_cat.
  • Complete equation to make more robust and include more parameters with degradation and transcriptional activity.
  • Take note about the double action of Luciferase, Luminescence and quiral activities.


To get unidirectionality we purpose a system with optical fiber and aisolated systems.

Image:Model.4.1.May.2010.jpg

Each beaker is coated by aluminium to focus photons to exit. This design is simple, we will change datails in sizes of elements and positions.

Wet lab

  • Insert your content here.



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