IGEM:UNAM Genomics Mexico/2009/Notebook/Wifi coli/2010/05/13: Difference between revisions
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We´ve got the primary function for activity of Luciferase and concentration of cytosolic Luciferin. | We´ve got the primary function for activity of Luciferase and concentration of cytosolic Luciferin. | ||
==Goals for next week== | |||
*Finish modeling of Luciferase activity. | *Finish modeling of Luciferase activity. | ||
*Start modeling of blue receptor. | *Start modeling of blue receptor. | ||
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*We need to determinate promoter strength for Luciferase with experimental data. To do that we | *We need to determinate promoter strength for Luciferase with experimental data. To do that we | ||
propose, an association between promoter and quantity of reporter. Search protocols with measure about light and concentration. | propose, an association between promoter and quantity of reporter. Search protocols with measure about light and concentration. | ||
TRpR → aλ[P1] | TRpR → aλ[P1] | ||
Promoter of Blue receptor → bλ[P2] | Promoter of Blue receptor → bλ[P2] | ||
Where λ is light. Ps, concentrations. | Where λ is light. Ps, concentrations. | ||
*Search buffer for best activity of Luciferase. (Claudia). | *Search buffer for best activity of Luciferase. (Claudia). |
Revision as of 17:14, 15 May 2010
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13th may 2010WetLabExtraction of genomic DNA for blue promoter is done, but we don´t have amplified. Goals for next week
ModelingWe´ve got the primary function for activity of Luciferase and concentration of cytosolic Luciferin. Goals for next week
Required data
propose, an association between promoter and quantity of reporter. Search protocols with measure about light and concentration. TRpR → aλ[P1] Promoter of Blue receptor → bλ[P2] Where λ is light. Ps, concentrations.
General
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