IGEM:UNAM Genomics Mexico/2009/Notebook/Wifi coli/2010/05/25: Difference between revisions
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Input Pops -> Photons. | Input Pops -> Photons. | ||
<math> | τ → ∞ -> <math>[14-CHO]=k</math> | ||
*This because the action of deg. and the production of CDE by M. acid tend to stabilize | *This because the action of deg. and the production of CDE by M. acid tend to stabilize | ||
Again we need to propose a formula: | Again we need to propose a formula: | ||
<math>[LuxAB]=Pops</math> | <math>[LuxAB]=Pops</math> | ||
===Pathway=== | ===Pathway=== | ||
<math>LuxAB-FMNH</math><sub>2</sub><math>-O</math><sub>2</sub><math>-RCHO -> LuxAB + FMN + R-COOH + H</math><sub>2</sub><math>O + Light</math> | |||
And the enzymes work in this form (Daniela has the references in German :S): | |||
<math>Acid M. -</math><sup>LuxD</sup><math>> C</math><sub>13</sub><math>H</math><sub>27</sub><math>-COOH -</math><sup>LuxE</sup><math>>C</math><sub>13</sub><math>H</math><sub>27</sub><math>Co-Amp -</math><sup>LuxC</sup><math>> 14-COOH</math> | |||
Here we see: | |||
*It appears that the product of the aldehyde can enter in the recicle pathway of LuxC and LuxE | |||
*We assume that C & E enzymes will be ocuppied with more substrate than the D, that only receives the membrane acid to form R-COOH. This mean, we need a major concentration of LuxCE than LuxD | |||
===Modelling with a Michael-Menten paradigm=== | ===Modelling with a Michael-Menten paradigm=== | ||
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*Formation of the dimer A|B | *Formation of the dimer A|B | ||
*Formation of the complex FMN-AB | *Formation of the complex FMN-AB | ||
*Formation of the complex R-COOH-FMN-AB | |||
*Temperature | *Temperature | ||
Can we assume Micahel-Menten equation? | Can we assume Micahel-Menten equation? | ||
Revision as of 01:17, 11 June 2010
 iGEM Project name 1
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25th May 2010RepresilatorA brief review to compare
A loop negative system.
Degradation of the mRNA, protein and the rate of transcription. Perspectives for our system
Limitants -> lifespan of E. coli?, luciferin?. "If is all on, we expect, that stuck there, but if one system turn off, maybe there is a stochastic component". LOVTAP System
Lifespan TetR
[math]\displaystyle{ δTetR= β - αTetRο }[/math] We require a formula: [math]\displaystyle{ F([TetR]) = Pops }[/math] But what happen with the production of endougenos TetR?, exists?.
Green emission
Input Pops -> Photons. τ → ∞ -> [math]\displaystyle{ [14-CHO]=k }[/math]
Again we need to propose a formula: [math]\displaystyle{ [LuxAB]=Pops }[/math] Pathway[math]\displaystyle{ LuxAB-FMNH }[/math]2[math]\displaystyle{ -O }[/math]2[math]\displaystyle{ -RCHO -\gt LuxAB + FMN + R-COOH + H }[/math]2[math]\displaystyle{ O + Light }[/math] And the enzymes work in this form (Daniela has the references in German :S): [math]\displaystyle{ Acid M. - }[/math]LuxD[math]\displaystyle{ \gt C }[/math]13[math]\displaystyle{ H }[/math]27[math]\displaystyle{ -COOH - }[/math]LuxE[math]\displaystyle{ \gt C }[/math]13[math]\displaystyle{ H }[/math]27[math]\displaystyle{ Co-Amp - }[/math]LuxC[math]\displaystyle{ \gt 14-COOH }[/math] Here we see:
Modelling with a Michael-Menten paradigmPerspectivesIt is relevant?:
Can we assume Micahel-Menten equation? To find list
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