IGEM:UNAM Genomics Mexico/2009/Notebook/iGEM 2011/2011/05/10
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χρόνος πέρασμα May 10th 2011
-We have skipped the steps 11, 13-20. -The centrifugations were performed under room temperature instead of 4° Celcius. -The step 4 was followed by TE 10:1 wash. -The final step (24) does not say the amount of TE-RNAse to be applied to the extracted plasmid. -Paz told me to use 20 microliter and let it rest at 37° for one hour prior to run the plasmids in an electrophoresis gel. -Uriel told us to use 60 microliters (we'll do it next time).
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