From OpenWetWareUniversity of Warsaw IGEM 2008 page
Welcome to University of Warsaw IGEM group page for year 2008
- Michał Krzysztoń
- Paweł Krawczyk
- Piotr Przanowski
- Ewa Szczęsna
- Łukasz Banasiak
- Kamil Lipiński
- Weronika Prusisz
- Marcin Piechocki
- Michał Lower
- prof. Jacek Bielecki
Monoclonal antibodies are proteins produced by cells of the immune system for defense against pathogens. Because of high target recognition specificity antibodies are object of research in many laboratories and pharmaceutical companies around the world. Currently exist numerous therapies based on monoclonal antibodies for diseases such as cancer, allergy, viral infections and autoimmunological diseases. Antibodies are also important research tool used to identify various chemical and biological particles.
Antibody producing GEM
Our research project is divided in three basic stages:
- In stage 1 we will introduce mechanism of targeted increase of genetical variability in regions of bacterial genome encoding antibodies. In this situation every bacterial cell in population will express different antibody on its surface.
- Goal of stage 2 is to provide selection mechanism, which will cause survival of the cells only when expressed antibody binds with target protein. Selection mechanism is based on antibiotic resistance induced by binding of target protein to antibody on cell surface.
- In stage 3 cellular metabolism will be switched to efficient antibody production and export.
Antibody will be then purified from culture medium. At all stages we have planed various control experiments, which will allow us to determine our progress.
This is very simplified description of our ‘biological machine’. Success of our project would allow simple, fast and cheap production of antibodies with desired specificity.