IGEM:UW/2008/Notebook/iGEM UW Team/2008/05/12: Difference between revisions

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1. Digestion of pTrc99A-AID with HindIII and NcoI.
1. Restriction digest of pTrc99A-AID with HindIII and NcoI.


2. DNA electrophoresis and isolation of DNA encoding AID from proper band.
2. DNA electrophoresis and isolation of DNA encoding AID from proper band.


3. Digestion of pMPM-T5 with HindIII and NcoI, use of alkaline phosphatase (CIAP).
3. Restriction digest of pMPM-T5 with HindIII and NcoI, use of alkaline phosphatase (CIAP).


4. DNA electrophoresis and isolation of pMPM-T5 backbone from proper band.
4. DNA electrophoresis and isolation of pMPM-T5 vector from proper band.


5. DNA ligation (AID + pMPM-T5 backbone)
5. DNA ligation (AID + pMPM-T5 backbone)

Revision as of 02:34, 12 June 2008

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1. Restriction digest of pTrc99A-AID with HindIII and NcoI.

2. DNA electrophoresis and isolation of DNA encoding AID from proper band.

3. Restriction digest of pMPM-T5 with HindIII and NcoI, use of alkaline phosphatase (CIAP).

4. DNA electrophoresis and isolation of pMPM-T5 vector from proper band.

5. DNA ligation (AID + pMPM-T5 backbone)


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