IGEM:UW/2008/Notebook/iGEM UW Team/2008/05/12

From OpenWetWare

(Difference between revisions)
Jump to: navigation, search
Line 14: Line 14:
4. DNA electrophoresis and isolation of pMPM-T5 vector from proper band.
4. DNA electrophoresis and isolation of pMPM-T5 vector from proper band.
-
5. DNA ligation (AID + pMPM-T5 vector)
+
5. DNA ligation (pMPM-T5+AID vector)
 +
 
 +
[[Image:UW_AID.jpg|150px]]
<!-- ## Do not edit below this line unless you know what you are doing. ## -->
<!-- ## Do not edit below this line unless you know what you are doing. ## -->

Revision as of 14:36, 12 June 2008

University of Warsaw iGEM Team 2008 Main project page
Previous entry      Next entry

1. Restriction digest of pTrc99A-AID with HindIII and NcoI.

2. DNA electrophoresis and isolation of DNA encoding AID from proper band.

3. Restriction digest of pMPM-T5 with HindIII and NcoI, use of alkaline phosphatase (CIAP).

4. DNA electrophoresis and isolation of pMPM-T5 vector from proper band.

5. DNA ligation (pMPM-T5+AID vector)


Personal tools