IGEM:UW/2008/Notebook/iGEM UW Team/2008/05/12

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5. DNA ligation (pMPM-T5+AID vector)
5. DNA ligation (pMPM-T5+AID vector)
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Revision as of 13:36, 12 June 2008

University of Warsaw iGEM Team 2008 Main project page
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1. Restriction digest of pTrc99A-AID with HindIII and NcoI.

2. DNA electrophoresis and isolation of DNA encoding AID from proper band.

3. Restriction digest of pMPM-T5 with HindIII and NcoI, use of alkaline phosphatase (CIAP).

4. DNA electrophoresis and isolation of pMPM-T5 vector from proper band.

5. DNA ligation (pMPM-T5+AID vector)


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