IGEM:UW/2008/Notebook/iGEM UW Team/2008/05/19: Difference between revisions
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1. PCR | 1. PCR - AID for translation fusion | ||
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2. PCR | 2. PCR - T7 RNA-polimerase for translation fusion | ||
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3. PCR | 3. PCR - T7 RNA-polimerase for transcription fusion | ||
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Revision as of 01:27, 11 June 2008
 University of Warsaw iGEM Team 2008
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1. PCR - AID for translation fusion Starters: AIDlNrH AIDpLinB Matrica: pTrc99A-AID Annealing temperature: 55 °C Annealing time: 60 sec 20 cycles
Starters: T7lLinkB T7pXbSal Matrica: E. coli Rosetta genomic DNA Annealing temperature: 62 °C Annealing time: 4 minutes 20 cycles
Starters: T7lRBSHi T7pXbSal Matrica: E. coli Rosetta genomic DNA Annealing temperature: 62 °C Annealing time: 4 minutes 20 cycles | |
