IGEM:UW/2008/Notebook/iGEM UW Team/2008/05/19

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Line 12: Line 12:
AIDpLinB
AIDpLinB
-
Matrica:
+
Template DNA:
pTrc99A-AID
pTrc99A-AID
Line 30: Line 30:
T7pXbSal
T7pXbSal
-
Matrica:
+
Template DNA:
E. coli Rosetta genomic DNA
E. coli Rosetta genomic DNA
Line 48: Line 48:
T7pXbSal
T7pXbSal
-
Matrica:
+
Template DNA:
E. coli Rosetta genomic DNA
E. coli Rosetta genomic DNA

Revision as of 04:21, 12 June 2008

University of Warsaw iGEM Team 2008 Main project page
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1. PCR - AID for translation fusion

Starters: AIDlNrH AIDpLinB

Template DNA: pTrc99A-AID

Annealing temperature: 55 °C

Annealing time: 60 sec

20 cycles


2. PCR - T7 RNA-polimerase for translation fusion

Starters: T7lLinkB T7pXbSal

Template DNA: E. coli Rosetta genomic DNA

Annealing temperature: 62 °C

Annealing time: 4 minutes

20 cycles


3. PCR - T7 RNA-polimerase for transcription fusion

Starters: T7lRBSHi T7pXbSal

Template DNA: E. coli Rosetta genomic DNA

Annealing temperature: 62 °C

Annealing time: 4 minutes

20 cycles


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