IGEM:UW/2008/Notebook/iGEM UW Team/2008/05/20: Difference between revisions
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2. PCR - translation fusion: AID + T7 RNA-polimerase | 2. PCR - translation fusion: AID + T7 RNA-polimerase | ||
Primers: | |||
AIDlNrH | AIDlNrH and | ||
T7pXbSal | T7pXbSal | ||
Revision as of 02:39, 12 June 2008
 University of Warsaw iGEM Team 2008
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1. Gel electrophoresis (yesterday PCR's) and DNA isolation from proper bands. 2. PCR - translation fusion: AID + T7 RNA-polimerase Primers: AIDlNrH and T7pXbSal Template DNA: AID for translation fusion and T7 RNA-polimerase for translation fusion Annealing temperature: 55 °C Annealing time: 4 minutes 20 cycles 3. Gel electrophoresis and DNA isolation from proper band (translation fusion: AID + T7 RNA-polimerase) | |
