IGEM:UW/2008/Notebook/iGEM UW Team/2008/05/23: Difference between revisions
From OpenWetWare
Jump to navigationJump to search
No edit summary |
No edit summary |
||
| (One intermediate revision by the same user not shown) | |||
| Line 28: | Line 28: | ||
11. Transformants plating on LB + tetracycline | 11. Transformants plating on LB + tetracycline | ||
[[Image: UW_A+T.jpg|350px]] | [[Image: UW_A+T.jpg|350px]] [[Image: UW_AT.jpg|350px]] | ||
Revision as of 11:42, 12 June 2008
 University of Warsaw iGEM Team 2008
|
<html><img src="/images/9/94/Report.png" border="0" /></html> Main project page <html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html> </html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html> |
|
1. Digest of pMPM-T5 with NcoI and XbaI 2. Digest of PCR product for translation fusion (AID+T7 RNA-polimerase) with NcoI and XbaI 3. Clean-up of digested products from p.1 and p.2 4. Ligation of digested products from p.1 and p.2 5. Transformation of E.coli TOP10 with ligation product 6. Digest of pMPM-T5+AID with HindIII and SalI 7. Digest of PCR product for transcription fusion (T7 RNA-polimerase) with HindIII and SalI 8. Clean-up of digested products from p.6 and p.7 9. Ligation of digested products from p.6 and p.7 10. Transformation of E.coli TOP10 with ligation product 11. Transformants plating on LB + tetracycline
| |
