IGEM:UW/2008/Notebook/iGEM UW Team/2008/05/23

From OpenWetWare

(Difference between revisions)
Jump to: navigation, search
Line 27: Line 27:
11. Transformants plating on LB + tetracycline
11. Transformants plating on LB + tetracycline
 +
 +
[[UW_A+T.jpg|350px]]
 +
<!-- ## Do not edit below this line unless you know what you are doing. ## -->
<!-- ## Do not edit below this line unless you know what you are doing. ## -->

Revision as of 14:40, 12 June 2008

University of Warsaw iGEM Team 2008 Main project page
Previous entry      Next entry

1. Digest of pMPM-T5 with NcoI and XbaI

2. Digest of PCR product for translation fusion (AID+T7 RNA-polimerase) with NcoI and XbaI

3. Clean-up of digested products from p.1 and p.2

4. Ligation of digested products from p.1 and p.2

5. Transformation of E.coli TOP10 with ligation product

6. Digest of pMPM-T5+AID with HindIII and SalI

7. Digest of PCR product for transcription fusion (T7 RNA-polimerase) with HindIII and SalI

8. Clean-up of digested products from p.6 and p.7

9. Ligation of digested products from p.6 and p.7

10. Transformation of E.coli TOP10 with ligation product

11. Transformants plating on LB + tetracycline

350px



Personal tools