IGEM:UW/2008/Notebook/iGEM UW Team/2008/06/03: Difference between revisions
From OpenWetWare
Jump to navigationJump to search
No edit summary |
No edit summary |
||
| Line 13: | Line 13: | ||
3. Digestion of pMPM-T5 + AID with HindIII and SalI | 3. Digestion of pMPM-T5 + AID with HindIII and SalI | ||
4. Clean-up products of p. | 4. Clean-up products of p.2 and p.3 reactions | ||
5. Ligation of p. | 5. Ligation of p.4 products (pMPM-T5 + AID transcription fusion with AID-T7 RNA-polimerease translation fusion) | ||
6. Transformation of E.coli TOP10 with ligation product | 6. Transformation of E.coli TOP10 with ligation product | ||
| Line 29: | Line 29: | ||
11. Isolation from proper band | 11. Isolation from proper band | ||
12. Ligation of product p. | 12. Ligation of product p.11 (pMPM-T5 + T7 RNA-polimerase) | ||
13. Transformation of E.coli TOP10 with ligation product | 13. Transformation of E.coli TOP10 with ligation product | ||
Revision as of 02:52, 11 June 2008
 University of Warsaw iGEM Team 2008
|
<html><img src="/images/9/94/Report.png" border="0" /></html> Main project page <html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html> </html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html> |
|
2. Digestion with HindIII and SalI 3. Digestion of pMPM-T5 + AID with HindIII and SalI 4. Clean-up products of p.2 and p.3 reactions 5. Ligation of p.4 products (pMPM-T5 + AID transcription fusion with AID-T7 RNA-polimerease translation fusion) 6. Transformation of E.coli TOP10 with ligation product 7. Transformants onto LB+tetracycline 8. Digestion of pMPM-T5 + transcription fusion with EcoRHI and HindIII 9. Use of T4 polimerase for blunting 10. Gel electrophoresis 11. Isolation from proper band 12. Ligation of product p.11 (pMPM-T5 + T7 RNA-polimerase) 13. Transformation of E.coli TOP10 with ligation product 14. Transformants onto LB + tetracycline
| |
